Karishma has been busily recording HSQC spectra for a whole set of protein-peptide interactions we’re looking at (let’s see – there are 18 peptides and 6 proteins – so she has about 108 titrations to do if we go the whole hog). So far, a decent proportion of them have been exceptionally good – like […]
The long-awaited structure of the double ZF of GATA1 bound to DNA – a pseudopalindromic site – has come out [Pubmed or PDF via journal] in Protein Science, thanks to the work of Lorna (including some *very* funky multi-coloured gel shifts), Krystal and Nina – and of course the inimitable David Jacques and Mitchell. Their […]
Look what turned up in a lab cleanup a little while ago. What is the probability of finding a gel that has lanes on it from both Jacqui *and* Joel being at the bench at the same time?!?! See it with your own eyes! I would note that the lanes with the best expression were […]
One of our most prized structures, the GATA:FOG zinc finger complex, features in the Protein Data Bank’s Molecule of the Month section for March 2007. Check it out! A couple of our recent reviews of the field (Sunde and Matthews and Gamsjaeger et al.) were also used to create the feature.
After about 7 years of painstaking work (!), we have finally been able to solve and publish the structure of the complex formed between the N-terminal zinc finger of GATA-1 and a classical finger from FOG. This work was made very difficult by the weak nature of the complex (~104-105 M-1), which presented line-broadening problems. […]
The structure of the complex formed between full-length LMO4 and the LMO4-binding region of ldb1 was published recently in EMBO J. This structure revealed a very unusual binding mode – a tandem beta-zipper, in which the ldb1 forms additional beta-strands to add on to hairpins in both of the LIM domains. The structure provides an […]