Protein structure, function and engineering

Beautiful, but CruEL…

Dec 8, 2020

Well, it’s one of those classic structural biology stories… Scientist (Anjumara) purifies and crystallizes protein, gets 3-A dataset at synchrotron (with Chandrika’s help). Can’t be solved, but scientists ask cryoEM colleagues kindly and they acquire some images (thanks Alastair and James!). Images are exquisite and quickly yield a 3-4 A map…..of GroEL. Scientists then have a good model for molecular replacement, and – lo and behold – can now solve crystal structure…. of GroEL…

Hard not to admire the beauty of the structure though!