MACKAY & MATTHEWS LAB

Protein structure, function and engineering

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Super-duper HSQC titration data

Karishma has been busily recording HSQC spectra for a whole set of protein-peptide interactions we're looking at (let's see - there are 18 peptides and 6 proteins - so she has about 108 titrations to do if we go the whole hog). So far, a decent proportion of them have been exceptionally good - like these! An embarrassment of riches...

Taylor Goes to New Zealand!

Over the holiday shutdown period, Taylor jumped over the Tasman for her first New Zealand visit (in the 11th hour of her PhD of course). It was an 8 day solo adventure with a jam packed schedule. One night in Auckland, 2 days in Rotorua, a 9 hour train across the North Island to Wellington for two days, then three days in Te Anau (with an excursion to Milford Sound), and capping it all off with a night in Christchurch. She saw gorgeous sights, read 5 books, relentlessly spammed everyone’s Facebook feeds with photos, and enjoyed the cooler temperatures before returning to a 45-degree Sydney. Ready to start that thesis?   Enjoy a few of the hundreds of photos...

Undiscovered Sydney – discovered (with Dave Gell)

Dave Gell was visiting from U Tasmania this week to determine the structure of a protein he has been working on for some time. Mario and Henry had kindly purified some protein for Dave - who's been busy with other work - and they grew some *beautiful* and very stout looking crystals. With James Walshe's help, they collected about three tons of data and then Mitchell guided them through the structure determination process (what a team effort!). A particularly cute feature of the process was that they solved the structure using anomalous signal obtained from iron atom in the protein - which can actually be observed on our home source. Who needs one of those synchrotron things? The data...

Have been off X for months but had to log in to see response to AF3. I am much less smart than the authors - but am I (and Erin) the only ones to think that the helical afros that it makes out of IDRs is totally weird? Am I missing something?

Amazing story from the lab down the corridor - @RezwanSidd working with Sandro Ataide and Ruth Hall in @SydneySOLES at @Sydney_Science - now they will be thrown into the gladiators' pit I suspect with the other protagonists that prowl this landscape! 🙂 Great piece of work!

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