{"id":4705,"date":"2020-12-04T16:51:21","date_gmt":"2020-12-04T05:51:21","guid":{"rendered":"http:\/\/mackaymatthewslab.org\/wp\/?p=4705"},"modified":"2020-12-04T16:51:21","modified_gmt":"2020-12-04T05:51:21","slug":"the-fog-tacc3-complex","status":"publish","type":"post","link":"https:\/\/mackaymatthewslab.org\/wp\/blog\/2020\/12\/04\/the-fog-tacc3-complex\/","title":{"rendered":"The FOG-TACC3 complex"},"content":{"rendered":"<p>[\u00a0<a href=\"http:\/\/www.rcsb.org\/pdb\/explore.do?structureId=1SRK\" target=\"_blank\" rel=\"noopener noreferrer\">PDB file<\/a>\u00a0] [\u00a0<a href=\"http:\/\/www.ncbi.nlm.nih.gov\/entrez\/query.fcgi?cmd=Retrieve&amp;db=pubmed&amp;dopt=Abstract&amp;list_uids=15234987\" target=\"_blank\" rel=\"noopener noreferrer\">PubMed link<\/a>\u00a0]<\/p>\n<p>FOG-1 is vital for the development of red blood cells and platelets. Originally identified as a Friend of GATA, GATA-independent functions are now emerging. Recently, we identified TACC3 as a protein partner of FOG-1. FOG-1 uses its third (of nine) ZnF to bind the coiled-coil domain of TACC3. The structure of FOG-1 finger 3 (left) shows that it is a typical classical CCHH finger (BMRB 6216). NMR and mutagenesis data identified the residues important for the interaction between FOG-1 (yellow) and TACC3 (blue) and this allowed us to use\u00a0<a href=\"http:\/\/www.nmr.chem.uu.nl\/haddock\/\">HADDOCK<\/a>\u00a0to calculate the structure of the FOG-TACC3 complex (right).<\/p>\n","protected":false},"excerpt":{"rendered":"<p>[\u00a0PDB file\u00a0] [\u00a0PubMed link\u00a0] FOG-1 is vital for the development of red blood cells and platelets. Originally identified as a Friend of GATA, GATA-independent functions are now emerging. Recently, we identified TACC3 as a protein partner of FOG-1. FOG-1 uses its third (of nine) ZnF to bind the coiled-coil domain of TACC3. The structure of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":4649,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":false,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[35],"tags":[],"class_list":["post-4705","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-structure-gallery"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/i0.wp.com\/mackaymatthewslab.org\/wp\/wp-content\/uploads\/2020\/12\/1SRK.png?fit=1800%2C1800&ssl=1","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/posts\/4705","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/comments?post=4705"}],"version-history":[{"count":1,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/posts\/4705\/revisions"}],"predecessor-version":[{"id":4716,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/posts\/4705\/revisions\/4716"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/media\/4649"}],"wp:attachment":[{"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/media?parent=4705"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/categories?post=4705"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/mackaymatthewslab.org\/wp\/wp-json\/wp\/v2\/tags?post=4705"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}