by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] The two zinc fingers of ZRANB2 (formerly known as ZNF265) can bind to single-stranded RNA with high sequence specificity. In addition to determining the structures of these two domains, we have used a combination of chemical shift mapping and mutagenesis...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] We used intein technology to generate a circular protein complex between LMO4 and the LIM-binding domain of Ldb1 (Ldb1-LID). The proteins, which bind in a head-to-tail fashion are joined by a flexible linker at each end. The circular...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] In developing motor neurons Isl1 displaces Lhx3 as the binding partner of the essential LIM-HD cofactor protein Ldb1. Isl1 provides Lhx3 with a decoy binding domain from a region in the C-terminus of Isl1 that, despite very low sequence...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] In developing V2 interneurons (and various other tissues) the LIM domains of Lhx3 must contact the LIM-interaction domain of Ldb1 as part of a transcription complex that specifies the cell type. This is how they do it. The Lhx3 is in blue...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] We have used a variety of NMR, mutagenesis and binding data to build a model of the interaction between zinc finger 5 from the neuronal transcription factor MyT1 and its cognate DNA site. The model was built using the HADDOCK data-driven...