MACKAY & MATTHEWS LAB

Protein structure, function and engineering

The LMO2:LDB1:GATA1:FOG1 complex

The LMO2:LDB1:GATA1:FOG1 complex

[ PDB file ] [ PubMed link ] All GATA1-activated genes are co-occupied by LMO2, LDB1 and a number of other proteins. Here, we have used a range of NMR and mutagenesis data to create a model of the complex formed by LMO2, LDB1 and the GATA1 N-finger. Surprisingly, the...
A truncated TIM barrel

A truncated TIM barrel

[ PDB file ] [ PubMed link ] The (�a)8 barrel is one of the most common protein folds and it is thought it was one of the very first protein folds to emerge. Our collaborator Wayne Patrick previously isolated a three-quarter barrel that is soluble and almost as...
The first PHD domain of CHD4

The first PHD domain of CHD4

[ PDB file ] [ PubMed link ] The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for the normal regulation of gene expression in a wide range of organisms (even plants!). We are slowly trying to build up a picture of how NuRD works at a mechanistic...
The FOG1-RbAp48 complex

The FOG1-RbAp48 complex

[ PDB file ] [ PubMed link ] FOG1 is a coregulator protein that assists the transcription factor GATA1 in the control of gene expression during erythroid development. Gerd Blobel, a collaborator of ours in Philadelphia, showed that at least part of the mechanism by...