by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] This is a second minimalised version of the CHANCE domain. This time, 13 of the 25 residues have been mutated (mostly to alanine), and the fold is retained. These two stripped down domains retain only the zinc-binding residues and the...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] This is a minimalised version of the CHANCE domain. 12 of the 25 residues have been mutated (mostly to alanine), but the fold is retained. This ‘stripping down’ of the surface of the domain is the first step in re-designing the...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] In order to get an idea of the conformation of SS3 at a sub-zero temperature, we determined the structure of SS3 at -5 degrees Celcius. Getting the solution down to that temperature without freezing was straighforward – slow cooling...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] [ PubMed link ] Many animals that live in extremely cold climates produce proteins that act to prevent the formation of ice crystals in the bodily fluids of the organism. Antarctic fish produce a range of such proteins, as the water temperatures can drop...
by Joel Mackay | Dec 4, 2020 | Structure Gallery |
[ PDB file ] This structure shows a two-residue insertion mutant of BKLF F3 that possesses native function but not native structure. This zinc finger retains the ability to bind to GATA-1, even though it contains two additional bulky residues in a structurally...